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pcdna3 1 þ vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcdna3 1 þ vector
    Pcdna3 1 þ Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+%C3%BE+vector/pm37354862-62-6-8
    Average 86 stars, based on 1 article reviews
    pcdna3 1 þ vector - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: Exploration of target molecules for prostate cancer gene therapy.
    Article Snippet: BACKGROUND.. Focusing on Adv-FZ33, a modified adenovirus in which a synthetic 33amino-acid immunoglobulin G-binding domain was inserted into the adenoviral fiber protein, we tried to identify suitable target molecules for prostate cancer-specific gene therapy.. METHODS.

    Plasmid Preparation:

    Article Title: Exploration of target molecules for prostate cancer gene therapy.
    Article Snippet: BACKGROUND.. Focusing on Adv-FZ33, a modified adenovirus in which a synthetic 33amino-acid immunoglobulin G-binding domain was inserted into the adenoviral fiber protein, we tried to identify suitable target molecules for prostate cancer-specific gene therapy.. METHODS.

    Article Title: Possible biased analgesic of hydromorphone through the G protein-over β-arrestin-mediated pathway: cAMP, CellKey™, and receptor internalization analyses.
    Article Snippet: Human MOR (hMOR) cDNA (NM_000914) with/without a stop codon was amplified from a Flexi ORF clone (Promega). .. The amplified hMOR fragment, N-terminal HaloTag® (Kazusa DNA Research Institute, Chiba, Japan) or Flag-fused hMOR were introduced into a pcDNA3.1 (þ) vector (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Mutated polyadenylation signals for controlling expression levels of multiple genes in mammalian cells.
    Article Snippet: A set of mutated SV40 early polyadenylation signals (SV40pA) with varying strengths is generated by mutating the AATAAA sequence in the wild-type SV40pA.. They are shown to control the expression level of a gene over a 10-fold range using luciferase reporter genes in transient transfection assays.. The relative strength of these SV40pA variants remains similar under three commonly used mammalian promoters and in five mammalian cell lines.

    Article Title: <i>PPARγ</i> regulates <i>fabp4</i> expression to increase DHA content in golden pompano (<i>Trachinotus ovatus</i>) hepatocytes
    Article Snippet: N-3 long-chain (≥C20) PUFA (LC-PUFA) are vital fatty acids for fish and humans.. As amain source of n-3 LC-PUFA for human consumers, the n-3 LC-PUFA content of farmed fish is important.. Previously, we identified fatty acid-binding protein (fabp)-4 as a candidate gene for regulating the n-3 LC-PUFA content.

    Article Title: Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest.
    Article Snippet: .. The products were subcloned into the pcDNA3.1(þ) vector (Invitrogen) and named pcDNA3.1-HA-Cyclin B1 and pcDNA3.1-HA-AURKA. .. Various mutants were generated by using the Fast Mutagenesis System (TransGen Biotech, Bellevue, WA).

    Transfection:

    Article Title: Exploration of target molecules for prostate cancer gene therapy.
    Article Snippet: BACKGROUND.. Focusing on Adv-FZ33, a modified adenovirus in which a synthetic 33amino-acid immunoglobulin G-binding domain was inserted into the adenoviral fiber protein, we tried to identify suitable target molecules for prostate cancer-specific gene therapy.. METHODS.

    Amplification:

    Article Title: Possible biased analgesic of hydromorphone through the G protein-over β-arrestin-mediated pathway: cAMP, CellKey™, and receptor internalization analyses.
    Article Snippet: Human MOR (hMOR) cDNA (NM_000914) with/without a stop codon was amplified from a Flexi ORF clone (Promega). .. The amplified hMOR fragment, N-terminal HaloTag® (Kazusa DNA Research Institute, Chiba, Japan) or Flag-fused hMOR were introduced into a pcDNA3.1 (þ) vector (Life Technologies, Carlsbad, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: MALAT1 induced migration and invasion of human breast cancer cells by competitively binding miR-1 with cdc42.
    Article Snippet: Competitive endogenous messenger RNAs (ceRNAs) affect other RNAs transcription through competitively binding common microRNAs (miRNAs).. In this study we identified long non-coding RNA (lncRNA) MALAT1 can function as a ceRNA of cell division cycle 42 (cdc42) 30UTR in inducing migration and invasion of breast cancer cells via miR-1.. We found that miR-1 bound both MALAT1 and cdc42 30UTR directly.

    Clone Assay:

    Article Title: MALAT1 induced migration and invasion of human breast cancer cells by competitively binding miR-1 with cdc42.
    Article Snippet: Competitive endogenous messenger RNAs (ceRNAs) affect other RNAs transcription through competitively binding common microRNAs (miRNAs).. In this study we identified long non-coding RNA (lncRNA) MALAT1 can function as a ceRNA of cell division cycle 42 (cdc42) 30UTR in inducing migration and invasion of breast cancer cells via miR-1.. We found that miR-1 bound both MALAT1 and cdc42 30UTR directly.

    Article Title: Molecular cloning and characterization of STC1 gene and its functional analyses in yak (Bos grunniens) cumulus granulosa cells.
    Article Snippet: Cumulus granulosa cells (CGCs), an important type of ovarian somatic cells, carries out various functions related to oogenesis, follicular development, and steroidogenesis.. Studying the biological mechanisms involved in the development and function of CGCs makes a great contribution to understanding the reproductive regulation in female animals.. Stanniocalcin-1 (STC1) is an important Ca2þ-regulated glycoprotein hormone that exhibits high expression levels in ovaries.

    Construct:

    Article Title: MALAT1 induced migration and invasion of human breast cancer cells by competitively binding miR-1 with cdc42.
    Article Snippet: Competitive endogenous messenger RNAs (ceRNAs) affect other RNAs transcription through competitively binding common microRNAs (miRNAs).. In this study we identified long non-coding RNA (lncRNA) MALAT1 can function as a ceRNA of cell division cycle 42 (cdc42) 30UTR in inducing migration and invasion of breast cancer cells via miR-1.. We found that miR-1 bound both MALAT1 and cdc42 30UTR directly.

    Article Title: <i>PPARγ</i> regulates <i>fabp4</i> expression to increase DHA content in golden pompano (<i>Trachinotus ovatus</i>) hepatocytes
    Article Snippet: N-3 long-chain (≥C20) PUFA (LC-PUFA) are vital fatty acids for fish and humans.. As amain source of n-3 LC-PUFA for human consumers, the n-3 LC-PUFA content of farmed fish is important.. Previously, we identified fatty acid-binding protein (fabp)-4 as a candidate gene for regulating the n-3 LC-PUFA content.

    DNA Sequencing:

    Article Title: MALAT1 induced migration and invasion of human breast cancer cells by competitively binding miR-1 with cdc42.
    Article Snippet: Competitive endogenous messenger RNAs (ceRNAs) affect other RNAs transcription through competitively binding common microRNAs (miRNAs).. In this study we identified long non-coding RNA (lncRNA) MALAT1 can function as a ceRNA of cell division cycle 42 (cdc42) 30UTR in inducing migration and invasion of breast cancer cells via miR-1.. We found that miR-1 bound both MALAT1 and cdc42 30UTR directly.

    Recombinant:

    Article Title: <i>PPARγ</i> regulates <i>fabp4</i> expression to increase DHA content in golden pompano (<i>Trachinotus ovatus</i>) hepatocytes
    Article Snippet: N-3 long-chain (≥C20) PUFA (LC-PUFA) are vital fatty acids for fish and humans.. As amain source of n-3 LC-PUFA for human consumers, the n-3 LC-PUFA content of farmed fish is important.. Previously, we identified fatty acid-binding protein (fabp)-4 as a candidate gene for regulating the n-3 LC-PUFA content.

    Transformation Assay:

    Article Title: Molecular cloning and characterization of STC1 gene and its functional analyses in yak (Bos grunniens) cumulus granulosa cells.
    Article Snippet: Cumulus granulosa cells (CGCs), an important type of ovarian somatic cells, carries out various functions related to oogenesis, follicular development, and steroidogenesis.. Studying the biological mechanisms involved in the development and function of CGCs makes a great contribution to understanding the reproductive regulation in female animals.. Stanniocalcin-1 (STC1) is an important Ca2þ-regulated glycoprotein hormone that exhibits high expression levels in ovaries.



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